FCL-06Pending Lab Analysis
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Fecal Coliforms

Collect a 250mL water sample in a sterile container for laboratory membrane filtration (CFU/100mL).

Why this matters — One Health

Their presence suggests inputs from human or animal waste and therefore a potential exposure to enteric pathogens including pathogenic bacteria, and parasites. This makes them a reliable early warning signal for the potential presence of harmful pathogens such as E. coli O157, Salmonella, or viruses (e.g. norovirus causing gastroenteritis) and parasites associated with gastrointestinal diseases.

Source: Importance of indicator — VII. Fecal Coliforms (factsheet_text.txt)

Collection Protocol

Collect water sample in sterile container. Filter on selective growth medium. Incubate at warm temperatures. Count colonies as CFU/100mL.

Laboratory Protocol Required

Requires membrane filtration or enzyme-substrate culture testing in an accredited laboratory (Method 9222 / ISO 9308-1). Cannot be evaluated visually in the field.

You cannot determine the result in the field.

Deterministic assessment

Awaiting laboratory analysis
  1. ObservationField record contains sample ID, collection protocol and notes only — no measurement.input/FCL-06/sample
  2. RuleOfficial measurement (factsheet §VII): a known volume of water is filtered onto a selective growth medium; after incubation, visible colonies are counted and reported as colony-forming units per 100 mL (CFU/100 mL). qPCR and eDNA methods are also described.FCL-06/lab-pending
  3. SourcesFactsheet §VII (Fecal Coliforms): colony counts "expressed as colony-forming units per 100 mL (CFU/100 mL)"; presence "suggests inputs from human or animal waste and therefore a potential exposure to enteric pathogens" (OneAquaHealth Key Indicators Factsheets, doi:10.5281/zenodo.20345207).source/factsheet/FCL-06
  • !We do not grade what we did not measure: no CFU/100 mL result exists until the laboratory reports one, so this indicator stays in pending state by rule.

Deterministic rules (streamvitals-assessment/1.0.0). No AI, no network, no hidden state.

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